FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l01n01_input_d.351000000655cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l01n01_input_d.351000000655cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8977320
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC503890.5612922342079819TruSeq Adapter, Index 7 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG106360.11847633814991558TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA62450.039.375231
TATGCCG40400.031.79928445
CAGTCAC122950.031.18302327
CCAGTCA123400.031.12400626
CCAGATC124450.030.95008333
TCCAGTC123450.030.94754225
AGTCACC125400.030.71698228
CATCTCG124400.030.67315339
GCACACG126650.030.57233611
CACACGT128400.030.27831312
ACACGTC130100.030.02101513
ACGTCTG129850.030.00950815
GTATGCC128750.030.0044545
CACGTCT130650.029.87741714
CAGATCA128400.029.87530134
ACTCCAG128050.029.84178423
CGTCTGA131050.029.7175516
CTCCAGT129300.029.58925224
AGCACAC131500.029.44476510
CGTATGC131200.029.44382544
Produced by FastQC (version 0.11.2)