FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l01n01_d2.35100000065661.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l01n01_d2.35100000065661.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7770228
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG381140.4905132770878795TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG78700.037.8502935
GTCCCGA79250.037.70116436
ACCCGTC82450.036.23792632
TCCCGAT82750.036.21530537
TCACCCG82700.036.04746230
CCCGATC82650.035.98690438
CGATCTC83200.035.8301440
CACCCGT83650.035.66428431
CCGATCT83600.035.4972339
GATCTCG84300.035.25584841
GCACACG86800.035.14761711
CACACGT87750.034.767112
ACACGTC87650.034.72976313
ACGTCTG87850.034.47142415
CACGTCT88000.034.43823614
AGCACAC88400.034.3842110
TCCAGTC89050.033.62922325
GAGCACA90650.033.5307669
ATCTCGT89200.033.4200442
GATCGGA58050.033.2794761
Produced by FastQC (version 0.11.2)