FastQCFastQC Report
Thu 14 Jul 2016
HFCJ7BCXX_l01n01_d1.351000000655f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFCJ7BCXX_l01n01_d1.351000000655f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8621609
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC496160.5754842280599828TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA72300.034.4157261
GCACACG121100.032.08527411
ACACGTC121050.032.04276713
CCTTGTA118650.032.00827433
CAGTCAC119300.031.89230327
TCCAGTC120100.031.73717525
CACACGT122750.031.59899712
ACGTCTG122500.031.57165315
CACGTCT123000.031.47989714
GAGCACA123500.031.334239
AGCACAC123650.031.33261110
AGTCACC121100.031.26962928
CCAGTCA122450.031.14538426
GTATGCC123900.030.94324945
CTCGTAT124400.030.70964842
CGTCTGA126550.030.61459716
TGTAATC124350.030.54106736
ACTCCAG125600.030.49373223
GAACTCC126850.030.38803121
ATCTCGT125800.030.31423640
Produced by FastQC (version 0.11.2)