FastQCFastQC Report
Tue 12 Dec 2023
HFC3NDRX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFC3NDRX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002673736
Sequences flagged as poor quality0
Sequence length10
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATTTCAA22382163022.322478585396997No Hit
AAGCGGAGGT22343803022.28422087641079No Hit
AAATCCCGCT15616018715.574376927730757No Hit
AATCTCACGC14035789313.99836137724465No Hit
GTGCTTATCA13773662413.736933466461117No Hit
GAGGAATCAG884845548.824860053978716No Hit
AGATCTCGGT56442200.5629169087959436Illumina Single End PCR Primer 1 (100% over 10bp)
GAGGACTCAG23861730.23798100162862948No Hit
GGGGGGGGGG19259080.19207723617884812No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)