FastQCFastQC Report
Thu 15 Aug 2024
HFC3LDRX5_l01_n01_AT082.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFC3LDRX5_l01_n01_AT082.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2486007
Sequences flagged as poor quality0
Sequence length99
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCTCGTTT221970.8928776145843516TruSeq Adapter, Index 16 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCTCGTAT144310.580489113667017TruSeq Adapter, Index 16 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142330.5725245343235156No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCTCGGTT134610.5414707199134999TruSeq Adapter, Index 16 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95540.38431106589804453No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCTCGGAT83430.3355984114284473TruSeq Adapter, Index 16 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCGCGGTT69530.27968545543113915TruSeq Adapter, Index 16 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCGCGTTT64200.25824545144080446TruSeq Adapter, Index 16 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCGCGGAT51110.20559073244765602TruSeq Adapter, Index 16 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCGCGTAT46950.18885707079666308TruSeq Adapter, Index 16 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCTCGTGT35540.142960176701031TruSeq Adapter, Index 16 (97% over 38bp)
GAAACTTACAAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAAGAGCCC32890.13230051242816293No Hit
CTCAAACTTCCGTGGCCTGGAAGGCCATAGTCCCTCTAAGAAGCTAGCTG26120.10506808709709989No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)