FastQCFastQC Report
Thu 15 Aug 2024
HFC3LDRX5_l01_n01_AT078.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFC3LDRX5_l01_n01_AT078.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4557589
Sequences flagged as poor quality0
Sequence length99
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGTAT1947374.272807398824247TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCGCGTAT839211.841346378534791TruSeq Adapter, Index 4 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGTATG63800.13998629538556462TruSeq Adapter, Index 4 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)