FastQCFastQC Report
Thu 15 Aug 2024
HFC3LDRX5_l01_n01_AT071.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFC3LDRX5_l01_n01_AT071.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25895905
Sequences flagged as poor quality0
Sequence length99
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGATTCATCTCGTAT1033240.39899744766595335TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGATTCATCGCGTAT494170.1908293994745501TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)