FastQCFastQC Report
Thu 15 Aug 2024
HFC3LDRX5_l01_n01_AT009.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFC3LDRX5_l01_n01_AT009.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17998206
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGGTTATCTCGTAT1364080.7578977593655724TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGGTTATCGCGTAT604580.3359112569330521TruSeq Adapter, Index 2 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291310.16185502043925934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)