FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_wt130_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_wt130_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13304404
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA234030.1759041592543341TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG43650.045.63204248
CGTATGC45050.044.51460646
GTATGCC49350.041.19979547
ATGCCGT51000.039.39498549
TCGTATG51000.039.38461345
GCACACG57350.036.80327211
CTCGTAT55300.035.7591344
ACGTCTG60050.035.09133515
TATCTCG58350.034.00071741
CACACGT62400.033.8250612
GCCGTCT63500.031.75058751
CACGTCT69650.030.35354414
TGCCGTC67300.030.2135350
ACACGTC70650.029.97295613
TCTCGTA68050.029.20949743
GTCACAT71450.028.6705729
AGCACAC77250.027.76879910
CCTTTAT75150.027.44955637
CAGTCAC80200.025.80055227
CCAGTCA84800.024.84825326
Produced by FastQC (version 0.11.2)