FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_wt130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_wt130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11890271
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG34450.042.98061846
CGTATGC36300.040.97410244
ACGTCTG51500.038.2388315
GTATGCC41250.036.8116945
GCACACG53950.036.5018511
ATGCCGT40450.034.98624447
CACACGT57550.034.39857512
ACACGTC57800.033.8917813
TCGTATG39800.033.46817843
CACGTCT59950.032.7339814
CTCGTAT45750.030.01848242
GTCACAT68400.028.28647429
TGCCGTC50000.028.23590948
AGCACAC72300.027.6667510
GCCGTCT49050.027.02378349
CGTCTGA74550.026.41593616
CATCTCG56950.025.86726839
CATGAGC76300.025.4482433
CAGTCAC75900.025.17308427
TCCAGTC77050.024.97651125
Produced by FastQC (version 0.11.2)