FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_wt115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_wt115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8636683
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG188480.21823193001294594TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG35900.051.24381346
CGTATGC36200.050.90844
ACTGACC39950.046.35766632
GTATGCC39700.046.33537345
ATGCCGT40150.046.08113547
TCGTATG41800.044.08702543
GCACACG46100.042.1178811
CACACGT46650.041.76989412
CTCGTAT43250.041.24962642
ACGTCTG47900.040.9692915
ACACGTC50150.038.7176613
CACGTCT51350.038.0821814
CACTGAC48750.038.059631
GCCGTCT49600.037.37202549
TGCCGTC51200.036.33814248
TCTCGTA50400.035.60005641
AGCACAC59150.033.2339310
CAGTCAC57350.032.83320227
GTCACTG58200.032.23535529
AGTCACT58550.032.2191828
Produced by FastQC (version 0.11.2)