FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_wt115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_wt115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8825145
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG161100.18254657572198532TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32600.050.92558346
CGTATGC35400.046.7942344
GTATGCC36850.045.3293545
TCGTATG38450.042.9891743
ATGCCGT39050.042.78285647
TAGGCAT43050.039.0955935
CTCGTAT42400.038.73858342
CACTTAG44350.038.57042331
TTAGGCA44400.038.3725134
ACGTCTG47150.038.1091915
ACTTAGG45050.037.97153532
GCACACG46950.037.90445711
CACACGT49600.036.0883312
GCCGTCT45800.035.57453549
TGCCGTC46900.035.1075848
ACACGTC51100.034.96149413
CACGTCT52500.034.02899614
TCACTTA52850.033.0848630
CATCTCG48650.032.90552539
TCTCGTA50400.031.9011541
Produced by FastQC (version 0.11.2)