FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_wt1030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_wt1030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9230339
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG147510.15980994847534852RNA PCR Primer, Index 29 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28150.046.9588346
CGTATGC28400.046.78446244
GTATGCC32900.040.59538745
ATGCCGT33100.039.939147
TCGTATG35550.037.27582643
ACGTCTG37100.037.1843815
GCACACG38000.036.5765711
CTCGTAT36950.035.02103442
CACACGT41950.033.21474512
ACACGTC42200.032.85452313
CACGTCT42250.032.65237814
GCCGTCT41800.031.54469749
TGCCGTC42650.031.23924348
AACTAAT43300.030.4275135
ACTAATC44200.029.02857436
CCAACTA48200.028.40666633
TCTCGTA45500.028.20927441
AGCACAC49800.027.84057810
AGTCACC50200.026.79342828
CGTCTGA53800.025.96240216
Produced by FastQC (version 0.11.2)