FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_wt1030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_wt1030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12118512
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG235750.19453708508107267RNA PCR Primer, Index 28 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG44050.050.9288746
CGTATGC46250.048.72575844
GTATGCC50400.044.64550845
ATGCCGT51050.044.28754447
TCGTATG53000.042.3883143
GCACACG55250.041.38582211
CTCGTAT56050.039.03407742
ACGTCTG59900.038.23095715
CACACGT61400.037.4656412
GCCGTCT62700.036.11522349
CACGTCT66750.034.3595814
ACACGTC68600.033.5333913
TGCCGTC69900.032.44391348
AGCACAC72350.032.2239310
TCTCGTA70800.031.09270541
AGTCACC75850.030.05459228
CAGTCAC78350.029.31569527
TCCAGTC79850.028.63530325
CCAGTCA80700.028.4192826
CTGAACT82550.028.0331719
Produced by FastQC (version 0.11.2)