FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_wt1015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_wt1015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9468766
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG318230.33608392054466235TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC53450.057.08128744
TATGCCG53750.056.6393646
GTATGCC56950.053.6952745
GTCACGC58650.052.09621429
TCGTATG59400.051.42083443
ATGCCGT59800.050.9684647
CACGCCA60400.050.70099331
ACGTCTG64550.048.29820315
AGTCACG63650.048.22130228
CTCGTAT62950.048.13417442
CGCCAAT64000.047.79745533
GCACACG65800.047.64299411
TCACGCC65100.046.93460530
CACACGT67650.046.4933112
TATCTCG65500.046.09438739
ACGCCAA67300.045.7077932
GCCGTCT67400.045.32489449
GCCAATA68300.044.68866734
ACACGTC71100.043.94649513
CACGTCT73200.042.68573814
Produced by FastQC (version 0.11.2)