FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_wt1015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_wt1015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8731686
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG215390.24667630054493483TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC45450.054.21450844
TATGCCG45750.053.71336446
GTATGCC48800.050.7765645
TCGTATG50000.048.31081843
ATGCCGT51200.047.86403347
GCACACG56800.047.72673411
ACGTCTG56800.047.6660115
CACACGT58550.046.4185712
CACGTCT60800.044.7007814
CACAGTG59300.043.4463633
CTCGTAT47050.043.12425642
ACACGTC63150.043.09194613
GCCGTCT56200.042.5630549
CACACAG61750.042.00088531
TGCCGTC62750.039.1101248
ACACAGT66100.039.080332
AGCACAC70550.038.6695810
GTCACAC68750.037.97525829
CAGTCAC69100.037.3838327
CCAGTCA70450.037.25492526
Produced by FastQC (version 0.11.2)