FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_wt060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_wt060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9628000
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCCG43300.045.31962632
CCCGAAT45700.042.03576735
GCACACG48900.041.61308711
ACGTCTG49700.041.01243615
CGTATGC26150.039.8559144
CACACGT52050.039.35996612
TATGCCG26750.039.3534946
ACACGTC53650.038.05776213
GTATGCC29300.037.45756545
CACGTCT54800.037.32204414
CTCCCGA53500.036.93767533
AGCACAC61750.033.5679110
CACTCCC60450.032.7474531
GTCACTC61650.032.3897429
AGTCACT65000.030.98602928
TCCAGTC66150.030.812325
CGTCTGA67350.030.46955516
CCAGTCA66200.030.26806326
CAGTCAC66050.030.12794127
ATGCCGT29250.030.09135647
Produced by FastQC (version 0.11.2)