FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_wt030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_wt030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9906663
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA358840.3622208608489054TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC63150.056.1856846
TATGCCG62950.056.14940648
GTATGCC67400.052.64467247
ATGCCGT68350.051.8650849
TCGTATG70550.050.48396345
ACGTCTG71900.050.46294415
GCACACG73700.049.27726411
CTCGTAT72200.048.51665544
TATCTCG73800.047.40654841
TGCCGTC75350.047.22982850
CACACGT77500.046.9058312
ACACGTC77800.046.6806313
CACGTCT79150.045.7972814
GCCGTCT77650.045.69867351
CACACTG81100.044.3115831
TCTCGTA81050.043.43039743
AGCACAC86400.042.2337710
CGTCTGA87100.041.69530516
GTCACAC86050.041.60246329
ACTGATA86950.041.25179734
Produced by FastQC (version 0.11.2)