FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_wt030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_wt030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8507488
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG187010.21981811787451241RNA PCR Primer, Index 24 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC36300.052.4792244
TATGCCG36850.051.3267846
GTATGCC40350.047.21346345
ATGCCGT41000.045.88084847
TCGTATG41500.045.40295843
ACGTCTG45450.044.46882615
AGTCACG43800.044.0171628
CGGTAGC44150.043.58906633
GCACACG45850.043.55380211
ACGGTAG46350.041.44570532
GGTAGCA46600.041.3723534
CACGGTA47900.040.104831
GTCACGG48250.039.95707329
ACACGTC50350.039.93567313
TGCCGTC47400.039.9058348
CACGTCT50400.039.82762514
CTCGTAT47250.039.21879242
CACACGT51150.039.17621612
GTAGCAT49350.038.57814435
GCCGTCT49500.037.8639849
Produced by FastQC (version 0.11.2)