FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_wt015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_wt015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14742642
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG215270.14601860372109693TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC44950.045.75794644
TATGCCG45050.045.355546
GTATGCC48800.042.5729845
ATGCCGT54850.037.31733347
GCACACG62150.036.7359511
ACGTCTG64550.035.58368715
TCGTATG56600.035.301643
CACACGT66300.034.64468412
CCGATGT64350.033.81822633
TATCTCG60750.032.7123139
ACACGTC70700.032.53736513
CGATGTA67800.032.0468534
CTCGTAT62250.031.8744842
CACGTCT74050.031.06548514
TGCCGTC70000.029.34110648
GCCGTCT68050.029.21859449
AGCACAC85150.027.38037710
GTCACCG82850.026.5995329
AGTCACC82950.026.52588728
TCTCGTA74800.026.52268641
Produced by FastQC (version 0.11.2)