FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ox6030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ox6030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10487653
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18100.029.36641746
CGTATGC19100.028.00667844
GCACACG29950.023.6064311
CACACGT30300.023.10610212
GTATGCC23950.022.33528745
ACGTCTG33400.021.58097315
TCGTATG25150.021.40619743
ATGCCGT24750.020.77986347
CGGGCGC15850.020.0184215
ACACGTC36200.019.53073513
CACGTCT36650.019.29102114
GGCGGGC16450.019.28835513
CGGAATA30350.019.09060734
GTGTGCG14500.018.0761728
AGCACAC40950.018.0232510
CGGCAAT33550.017.9907761
TATCTCG31850.017.54943739
CTCGTAT32000.017.25465842
CGCGTAT10000.016.9005341
GCGGGCG19600.016.18851914
Produced by FastQC (version 0.11.2)