FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ox6030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ox6030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8436304
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG99280.11768186637181402RNA PCR Primer, Index 36 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22850.042.5949346
CGTATGC23750.041.1222344
GTATGCC25750.037.9287245
ATGCCGT28250.034.82271647
TCGTATG28850.034.6886943
GCACACG30000.034.3738511
CACACGT32000.032.4410412
ACGTCTG34250.030.51127415
ACACGTC34500.029.79051613
CACGTCT37050.027.83324214
CTCGTAT34900.027.38841642
GTCACCC36100.027.1318329
TGCCGTC37100.026.5170548
GCCGTCT37650.026.31336249
AGCACAC44350.023.95164910
CGTCTGA45200.023.42464816
TCTCGTA41050.023.19793141
TCACCCA42800.022.88442430
AGTCACC45300.021.92640528
CACCCAA45950.021.61585431
Produced by FastQC (version 0.11.2)