FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ox6015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ox6015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8136617
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA203200.24973524008811035TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC35450.051.01880646
TATGCCG35750.050.59565448
GTATGCC38900.046.67461847
ATGCCGT40100.044.6769649
TCGTATG41250.043.75834745
GCACACG45250.042.83451511
CCGTATC43200.041.60954338
ACGTCTG46750.041.60743715
CGTATCT43350.040.82986539
CACACGT47650.040.6775612
CTCGTAT44350.040.46544
ACACGTC47950.040.35088313
TCCGTAT45800.040.29710837
TTCCGTA46350.040.10999336
TATCTCG45550.039.61603541
AGTTCCG48950.038.32854534
CACGTCT50650.038.26822714
TGCCGTC47750.037.736450
GCCGTCT49000.036.4937451
GTTCCGT52550.035.3094635
Produced by FastQC (version 0.11.2)