FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ox6015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ox6015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9619151
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA355370.36944008883944124TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG59750.055.74906548
CGTATGC60200.055.5541846
GTATGCC62650.053.33157347
ATGCCGT63950.052.1408449
TCGTATG65200.051.34255245
GCACACG69850.048.8835111
CACACGT71700.047.5746112
ACGTCTG71800.047.4125215
CTCGTAT71200.046.23872844
ACACGTC73350.046.22229813
CACGTCT76650.044.41274614
TGCCGTC75900.044.11509350
TCTCGTA75350.043.69093343
GCCGTCT77500.042.80524451
CACAGTC81600.041.37786531
AGCACAC84300.040.6270510
CGTCTGA85100.039.96121616
GTCACAG86900.039.013229
ACAGTCA87100.038.88381632
CAGTCAC87950.038.66547827
Produced by FastQC (version 0.11.2)