FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ox130_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ox130_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8697868
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG198840.2286077461741199RNA PCR Primer, Index 33 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGCG39200.055.2548633
TATGCCG39750.052.52908346
CGTATGC40100.052.14797244
CAGGCGA44000.048.208334
GTATGCC44500.047.4586745
ATGCCGT44450.046.9017747
GCACACG49000.045.96280311
TCGTATG45600.045.7798943
ACGTCTG52050.043.20298415
ACCAGGC50300.042.99163432
CACACGT52400.042.8490912
ACACGTC52450.042.6764813
AGGCGAT52050.040.15607535
TGCCGTC52700.039.82259448
GGCGATC48350.039.52097336
CACGTCT57000.039.39086514
CTCGTAT50200.038.1466142
AGCACAC60150.037.9596110
GCGATCT51300.036.98491337
GCCGTCT57000.036.2132849