Basic Statistics
Measure | Value |
---|---|
Filename | HF7VNBGX5_n01_ox130_2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8697868 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 19884 | 0.2286077461741199 | RNA PCR Primer, Index 33 (100% over 43bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCAGGCG | 3920 | 0.0 | 55.25486 | 33 |
TATGCCG | 3975 | 0.0 | 52.529083 | 46 |
CGTATGC | 4010 | 0.0 | 52.147972 | 44 |
CAGGCGA | 4400 | 0.0 | 48.2083 | 34 |
GTATGCC | 4450 | 0.0 | 47.45867 | 45 |
ATGCCGT | 4445 | 0.0 | 46.90177 | 47 |
GCACACG | 4900 | 0.0 | 45.962803 | 11 |
TCGTATG | 4560 | 0.0 | 45.77989 | 43 |
ACGTCTG | 5205 | 0.0 | 43.202984 | 15 |
ACCAGGC | 5030 | 0.0 | 42.991634 | 32 |
CACACGT | 5240 | 0.0 | 42.84909 | 12 |
ACACGTC | 5245 | 0.0 | 42.67648 | 13 |
AGGCGAT | 5205 | 0.0 | 40.156075 | 35 |
TGCCGTC | 5270 | 0.0 | 39.822594 | 48 |
GGCGATC | 4835 | 0.0 | 39.520973 | 36 |
CACGTCT | 5700 | 0.0 | 39.390865 | 14 |
CTCGTAT | 5020 | 0.0 | 38.14661 | 42 |
AGCACAC | 6015 | 0.0 | 37.95961 | 10 |
GCGATCT | 5130 | 0.0 | 36.984913 | 37 |
GCCGTCT | 5700 | 0.0 | 36.21328 | 49 |