FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ox130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ox130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8277758
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG379500.45845747121382385RNA PCR Primer, Index 32 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG61300.059.23332246
CGTATGC62950.057.61651244
GTATGCC65950.054.99758545
ATGCCGT66800.054.05154447
TCGTATG67600.053.70137443
GCACACG74350.050.56094711
CTCGTAT70400.049.94525542
CACACGT75400.049.8117112
ACGTCTG75300.049.5563615
ACACGTC78600.047.82763313
TGCCGTC76200.047.3866348
GCCGTCT76150.046.91937349
CACGTCT80350.046.57134614
TCTCGTA76700.045.43300641
AGCACAC85600.044.2382810
CCACTCA85300.043.18092333
CCAGTCA85600.042.8673926
CAGTCAC85800.042.8076627
AGTCACC86350.042.53500428
CGTCTGA88050.042.301816
Produced by FastQC (version 0.11.2)