FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ox115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ox115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11908190
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG150670.12652636546779988TruSeq Adapter, Index 10 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC36700.045.23095744
TATGCCG37150.045.0599646
GTATGCC41400.040.76463345
ATGCCGT43050.038.08659447
TCGTATG44300.035.75543243
GCACACG54500.034.80430211
ACGTCTG56800.033.5775515
CACACGT57000.033.27793512
TAGCTTA58350.032.50855634
TATCTCG51400.031.7486539
CACTAGC59900.031.55047431
CTCGTAT52350.030.5852842
TCACTAG62150.030.13081230
ACACGTC63250.030.09878213
CACGTCT64800.029.27250314
TGCCGTC58000.028.151648
TCTCGTA57350.028.09487341
GCCGTCT57050.027.65236949
GTCACTA72100.026.35544229
AGTCACT73300.026.2062728
Produced by FastQC (version 0.11.2)