FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ox115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ox115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12891727
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG333500.25869303623944256TruSeq Adapter, Index 9 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC60400.051.8776444
TATGCCG60750.051.4141346
GTATGCC65800.047.62187245
TCGTATG68100.046.3135943
ATGCCGT68750.045.48441747
AGTCACG72200.043.7571128
GCACACG74950.042.84253711
GTCACGA74050.042.71016729
ACGTCTG77900.041.1760115
CTCGTAT74750.041.1298742
CACACGT78650.040.7395112
TGCCGTC78600.040.18054648
GCCGTCT80550.039.3798449
ACACGTC82100.039.11157613
CACGTCT86450.037.38322414
TCTCGTA84600.036.09288841
AGCACAC94700.034.56301510
CGATCAG92000.034.22722233
CACGATC95450.033.4595731
CGTCTGA97300.033.2849716
Produced by FastQC (version 0.11.2)