FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ox1030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ox1030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8173229
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG126470.15473688550755155RNA PCR Primer, Index 35 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG26950.046.49294346
CGTATGC27250.045.84993444
GTATGCC30400.041.10029245
TCGTATG32500.038.54747843
ATGCCGT32700.038.21576747
GCACACG35200.038.01439711
CACACGT36600.037.12566412
ACGTCTG36800.036.82927315
CTCGTAT35750.035.04037542
TATCTCG36100.034.69363839
CACGTCT41050.032.76482414
ACACGTC41850.032.303913
TGCCGTC39900.030.97456448
AGCACAC46000.029.46414210
TCTCGTA42750.029.45862441
GCCGTCT41850.028.7890749
AGTCACC46750.028.47384828
CAGTCAC49600.027.04633327
CATTTTA49450.026.99005134
CGTCTGA51250.026.71437816
Produced by FastQC (version 0.11.2)