FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ox1030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ox1030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9598343
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG28450.020.24323511
CACACGT30100.019.13375512
ACGTCTG31150.018.04613715
ACACGTC31400.017.46295213
CGGGCGC7050.017.12117815
CACGTCT35550.016.10352514
GTGTGCG10250.014.1308698
GGCGGGC9050.014.09948813
AGCACAC42100.014.007688510
CGCGTAT7550.012.7909941
CGTCTGA44400.012.73848816
GCCAGCG19000.012.52433634
CGGACAT16800.012.5231551
GTCCTAT21100.012.10314443
CGCGGCT15200.012.0261181
CGGCAAT23650.011.9584781
GCGGGCG10150.011.89198914
AGTCACC44300.011.832931528
CGGGCAT13650.011.370331
CCTATAA20400.011.33503945
Produced by FastQC (version 0.11.2)