FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ox1015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ox1015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12420309
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG283660.2283840120241775TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC54500.052.17880244
TATGCCG55450.051.10389746
GTATGCC59350.047.91694645
ATGCCGT61800.045.79984347
TCGTATG62150.045.6428843
GCACACG67550.043.70385411
CACACGT70500.041.82618712
ACGTCTG72200.041.080215
ACACGTC75100.039.31019213
CTCGTAT70800.038.4554742
TGCCGTC76650.036.79262548
CACGTCT80600.036.67037614
GCCGTCT76950.036.42577749
GTCACCT79850.036.23692729
TCTCGTA79000.034.45834441
AGCACAC88150.033.9212310
AGTCACC85250.033.78015528
CGTCTGA90800.032.7410616
CAGTCAC92150.031.40047327
CCAGTCA93050.031.13382326
Produced by FastQC (version 0.11.2)