FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ox1015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ox1015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9568485
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG299040.3125259641416588TruSeq Adapter, Index 11 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC48800.055.3767744
TATGCCG49500.054.6013746
GTATGCC53200.051.05810545
ATGCCGT55050.049.22561347
TCGTATG56400.048.2191143
CGGCTAC60050.045.54176733
AGTCACG60450.045.1824228
GCACACG60950.045.15266811
ACGGCTA62350.043.97126832
CTCGTAT60900.043.74619342
CACGGCT62800.043.6008231
ACGTCTG63550.043.25221315
CACACGT64650.042.676112
GTCACGG64250.042.56360229
TGCCGTC64550.042.35618248
GGCTACA64450.042.00521534
ACACGTC66300.041.4581913
GCTACAT66300.040.57431435
CACGTCT68750.040.03114314
GCCGTCT67800.039.86863349
Produced by FastQC (version 0.11.2)