FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ox030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ox030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7316382
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG482550.6595473008380371RNA PCR Primer, Index 31 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG73150.061.7141446
CGTATGC74700.060.61094744
GTATGCC77100.058.635145
CACGATA78350.058.06110834
TCGTATG78750.057.4910643
ATGCCGT79250.056.8822547
GCACACG83850.054.9934311
ACGTCTG85550.054.3037815
TATCTCG81950.054.223339
CTCGTAT83400.053.53755642
CACACGT86850.053.21369612
ACGATAT85950.052.92714335
TGCCGTC84950.052.9041148
ACACGTC87750.052.8251313
CCACGAT86750.052.75638633
CACGTCT89500.051.79187814
GCCGTCT86700.051.51917349
TCTCGTA87700.050.7875941
AGCACAC94650.049.08281710
AGTCACC94750.048.41155228
Produced by FastQC (version 0.11.2)