FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ox030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ox030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8662323
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG42300.043.37796411
CACACGT45000.040.6986412
ACGTCTG45850.040.16888415
ACACGTC47300.038.93839313
ATCGGGG14500.037.35747540
CACGTCT50500.036.40250414
GATCGCG15750.033.9544739
AGCACAC55500.033.3098210
GATCGGG25500.031.25486639
CGTCTGA59450.031.0373616
AGTCACC57650.030.98887628
ACCGGAT56050.030.7671535
CGGATCG44900.030.50087437
CAGTCAC58250.030.4920527
CGGGGTG6850.029.73261351
TCCAGTC62800.029.2713625
CCGGATC58400.028.58514836
CCAGTCA63350.028.14616626
ACTCCAG65700.028.03165423
CTGAACT66200.027.97671119
Produced by FastQC (version 0.11.2)