FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ox015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ox015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9842091
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG297790.3025678181597793TruSeq Adapter, Index 8 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC59500.055.5089144
TATGCCG59700.055.10236446
GTATGCC63150.053.0137145
GCACACG83550.050.52327711
TCGTATG62350.050.14740443
ATGCCGT63100.050.00160647
ACGTCTG84650.049.7852615
CACACGT84800.049.6575312
ACACGTC87500.048.2831513
CACGTCT91450.046.08428614
CTCGTAT69600.045.1190742
GTCACAC94250.043.43206429
AGCACAC98700.043.22220210
CGTCTGA99900.042.28875716
TGCCGTC73400.042.1393648
TCTCGTA75150.041.69063641
CAGTCAC98550.041.53700327
GCCGTCT70300.041.49450749
TCCAGTC101600.041.30856725
CCAGTCA101250.040.56559426
Produced by FastQC (version 0.11.2)