FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ox015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ox015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11149521
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG251060.22517559274519505TruSeq Adapter, Index 7 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC49000.051.8377644
TATGCCG50400.049.9925846
GTATGCC53300.047.4629745
ATGCCGT54800.045.35117747
TCGTATG55600.043.75740443
GCACACG65550.043.67022711
CACACGT67950.042.2800612
ACGTCTG69250.041.38619215
ACACGTC69500.041.2380613
CTCGTAT61700.039.0376942
CACGTCT74000.038.7303414
TGCCGTC66900.036.8407348
GCCGTCT64800.036.54390749
CATCTCG68350.035.536639
AGCACAC82500.035.15783310
CGTCTGA85450.033.6207316
TCTCGTA71850.033.47028741
AGTCACC82600.033.27718728
CAGTCAC84950.032.27543327
ACTCCAG90650.031.38748223
Produced by FastQC (version 0.11.2)