FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ko6030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ko6030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9516320
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG124250.130565176454764RNA PCR Primer, Index 45 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC28750.044.1712244
TATGCCG29750.042.46117846
ATGCCGT33150.038.6300147
GTATGCC33750.037.42480545
GCACACG37850.036.26698711
ACGTCTG39250.035.41200615
TCGTATG36550.034.7433443
CACACGT40650.033.93858712
CTCGTAT38200.032.51912742
ACACGTC42450.032.2557513
TGCCGTC42700.029.90959748
CACGTCT46400.029.80685214
GCCGTCT45200.028.02694749
CGTCTGA51650.026.84360916
AGCACAC54550.025.79616410
TCTCGTA48400.025.66281741
CACTCAT50900.025.61211831
CATCTCG48850.025.42641639
GTCACTC52350.025.23224329
AGTCACT54950.024.22677828
Produced by FastQC (version 0.11.2)