FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ko6030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ko6030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10965283
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG153110.13963159911148668RNA PCR Primer, Index 44 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC32800.042.82292644
TATGCCG33450.041.89297546
GTATGCC38350.036.8969245
ATGCCGT39950.035.42485447
TCGTATG40450.035.31960343
GCACACG43550.033.8947211
CACTATA42250.033.6303331
ACGTCTG44700.032.63663515
CTATAAT45150.031.69968233
TATCTCG44550.031.44250939
CACACGT47550.031.11624512
CTCGTAT45600.030.87531942
ACACGTC48300.030.27604513
TATAATA48600.029.37923834
GCCGTCT49000.029.23552149
TGCCGTC50050.028.829148
CACGTCT51350.028.47762914
ACTATAA56200.025.71214532
GTCACTA57450.025.39305129
TCTCGTA56450.025.05917241
Produced by FastQC (version 0.11.2)