FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ko6015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ko6015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9516924
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA300130.31536450222782064TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG49200.055.2124648
CGTATGC50250.054.12147546
GTATGCC55100.049.48652647
ATGCCGT55100.049.1753949
TCGTATG56350.048.62483245
AGTCACG58050.047.41047728
ACGTCTG61700.046.17358815
GCACACG62250.045.8766811
ACGAGTG60900.044.96546632
GTCACGA61400.044.87991729
TATCTCG59600.044.8656541
CGAGTGG61600.044.51118533
CACGAGT62250.044.48871231
CTCGTAT60550.044.2242944
CACACGT66450.043.0806112
ACACGTC67850.042.0900313
GCCGTCT64750.041.95691351
CACGTCT68650.041.70002414
TGCCGTC65450.041.29503650
TCACGAG70300.039.19810530
Produced by FastQC (version 0.11.2)