FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ko6015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ko6015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10963271
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA208230.18993419026128242TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA40950.050.36778335
CGTACGT41400.049.3195434
CGTATGC42000.048.48301746
TATGCCG41850.048.1665948
CCGTACG44950.045.8837833
GTATGCC46500.043.497247
TACGTAA48050.042.6389536
GCACACG51050.042.15978211
TCGTATG49150.041.0049845
ATGCCGT50050.040.34449449
ACGTCTG53900.039.54552515
CACACGT54600.039.16576412
ACGTAAT54550.037.24582337
ACCGTAC56600.036.80427632
ACACGTC58300.036.62095313
CTCGTAT54200.036.60985644
CACGTCT60400.035.4046514
TGCCGTC57700.035.295650
CGTAATC59100.033.79959538
GCCGTCT60550.033.46540551
Produced by FastQC (version 0.11.2)