FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ko130_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ko130_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8284882
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG291900.3523284942380592RNA PCR Primer, Index 41 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC49550.055.16102244
TATGCCG50200.054.65764246
TCGTATG53000.051.5030643
GTATGCC54050.050.82545545
ATGCCGT54250.050.6438947
GCACACG59650.048.10566711
ACGTCTG59950.047.7510315
AGTCACG58800.047.7449928
CTCGTAT57100.047.2573142
GTCACGA59750.046.81269529
CACACGT63300.045.27813312
ACACGTC64650.044.38599813
TGCCGTC61850.044.08657548
ACGACAT62150.043.6739235
GACGACA63350.043.3900234
GCCGTCT63150.043.0160849
CACGTCT67750.042.25400514
TCTCGTA65600.041.12856341
TCACGAC68450.040.9635730
AGCACAC71550.040.2012710
Produced by FastQC (version 0.11.2)