FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ko130_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ko130_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11011250
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGATCCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA230930.20972187535475084TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC45600.048.66859446
TATGCCG45750.048.58954248
GTATGCC49850.044.93779847
TCGTATG52050.043.0331345
ATGCCGT52050.042.8421949
GTCACGT53650.041.91189229
GCACACG56800.041.35022411
AGTCACG55000.041.0716328
ACGTGAT55900.040.59527232
ACGTCTG57250.040.42222615
TCACGTG55700.040.2455230
CACGTGA57300.039.4228531
CACACGT59500.039.2423312
ACACGTC60800.038.34636313
CTCGTAT56250.037.73234644
CACGTCT64150.036.2363414
TGCCGTC61700.035.97538450
GCCGTCT62350.035.54610451
CGTGATC65500.034.48825533
AGCACAC72500.032.6811910
Produced by FastQC (version 0.11.2)