FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ko115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ko115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10260117
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC20950.044.93992233
CCGCACA23100.040.1637536
TATGCCG24800.038.2795448
TCCGCAC24850.037.8882735
CGTATGC24800.037.7192546
ACGTCCG26050.036.2737432
GTCCGCA26400.036.05497734
CGCACAT26000.035.2901137
GTATGCC28300.033.3002247
TCGTATG29150.032.08750545
ATGCCGT30350.031.39308549
GCACACG33000.030.93337211
ACGTCTG32650.030.41997115
AGTCACG31850.030.10156428
GTCACGT34600.028.00798229
CACACGT37750.026.5845812
ACACGTC38750.025.54265413
CTCGTAT37750.024.77665944
TCACGTC38750.024.74130430
CACGTCT41100.024.3336814
Produced by FastQC (version 0.11.2)