FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ko115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ko115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10319993
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG109360.10596906412630319RNA PCR Primer, Index 17 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC24650.038.22228244
TATGCCG25950.035.9118146
GTATGCC30050.031.12462645
ATGCCGT30700.030.35748347
TCGTATG31150.029.91327743
TCACGTA32300.029.46874830
GCACACG34800.028.24515211
GTCACGT33850.028.01748729
AGTCACG34800.027.25277328
ACGTCTG35700.026.75958315
ACGTAGA36000.025.86523432
CACGTAG36900.025.60809331
CACACGT38500.025.08265512
ACACGTC38750.024.83170713
CTCGTAT38250.024.63046542
TGCCGTC38950.024.1940348
CACGTCT42850.022.53623214
GCCGTCT42150.022.52167949
CGTAGAG42650.022.4796433
AGCACAC50850.019.8724210
Produced by FastQC (version 0.11.2)