FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ko1030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ko1030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5344296
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG18700.027.4823911
ACGTCTG18750.027.40884815
CGTATGC8650.026.72957644
CACACGT20450.025.2992612
TATGCCG9900.023.70409446
ACACGTC22900.023.19503813
TACAGCA22700.022.33579334
CACGTCT23500.022.30907214
CACTACA23200.022.30000531
GTATGCC11700.021.82625645
ACTACAG22950.021.79149232
CTACAGC23900.021.79118233
CGGGCGC5450.020.25159615
AGCACAC27400.019.51151510
CGTCTGA27150.019.1826616
CGCGTAT6300.019.16955842
GGCGGGC6100.018.6592413
GTCACTA27750.018.6436129
CAGTCAC28550.018.4836227
ATGCCGT10900.018.3645247
Produced by FastQC (version 0.11.2)