FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ko1030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ko1030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10357357
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG165150.1594518756088064RNA PCR Primer, Index 42 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC35100.047.39101844
TATGCCG35250.047.0957746
GTATGCC40750.040.9904145
ATGCCGT40800.040.86111547
TCGTATG41550.040.28171543
GCACACG44100.038.32062511
ACGTCTG45600.036.90987815
CTAATCG45500.036.6111133
CACACGT47750.035.53637312
ACACGTC50250.033.70008513
ACTAATC50100.033.2491332
CTCGTAT48500.033.1555542
TGCCGTC50250.033.04052748
GCCGTCT51650.032.2119349
CACTAAT53700.031.27748531
TAATCGA55200.030.05340434
CACGTCT56350.030.0521314
AGCACAC60000.028.1084510
TCTCGTA57000.027.90469641
GTCACTA61000.027.87367429
Produced by FastQC (version 0.11.2)