FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ko1015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ko1015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11369791
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA220380.19382942043525692TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG44500.048.79498748
CGTATGC44800.048.6165746
GTATGCC49500.044.2117847
ATGCCGT51350.042.6225249
GCACACG53000.042.55632811
TCGTATG51500.042.1531945
AGTCACG53950.041.35784528
GGCCTTA53550.041.3496836
ACGTCTG54800.041.2205215
ACGTGGC56150.039.85989832
CACACGT56900.039.69989812
GTCACGT56050.039.56219529
GCCTTAT57900.038.7240837
TCACGTG57600.038.6768730
ACACGTC59400.038.1449813
TATCTCG55950.037.92457641
CACGTGG59250.037.54158431
CTCGTAT58150.036.73776244
TGCCGTC60350.036.4402350
GCCGTCT60150.036.2195751
Produced by FastQC (version 0.11.2)