FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ko1015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ko1015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10269301
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA170720.16624305782837603TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC38850.043.8862746
TATGCCG38750.043.82757648
GTATGCC42800.039.9203547
GCACACG46200.039.19176511
ATGCCGT44350.038.45014649
TCGTATG44900.037.8159945
AGTCACG45950.037.60234528
GTCACGT47100.036.90390829
ACGTCTG48600.036.90118815
CACACGT49350.036.7600512
CACGTGA48950.035.2978131
TCACGTG49650.035.00853730
CTCGTAT47700.034.5827844
ACACGTC53000.034.09815213
GCCGTCT51950.032.82766351
CACGTCT55900.032.0825614
TGCCGTC53450.031.90499750
ACGTGAA56300.030.93498832
AGCACAC63000.029.50733410
TCTCGTA55800.029.31416943
Produced by FastQC (version 0.11.2)