FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ko030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ko030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10678281
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG242120.2267406148985965RNA PCR Primer, Index 39 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC44300.050.63796244
TATGCCG45800.048.6819846
GTATGCC48100.046.2797945
CACCTAT50400.044.3410431
TCGTATG50600.044.19434443
ACCTATA51500.043.5948532
ATGCCGT51150.043.38992747
ACGTCTG54350.042.19798315
TCACCTA53700.041.42350830
GCACACG55300.041.3489111
CCTATAC54400.041.3348433
CTATACA55800.039.74169534
CACACGT57850.039.34741612
ACACGTC59600.038.13439613
CTCGTAT57450.037.96162442
TGCCGTC60050.037.0183348
CACGTCT62200.036.5956314
GCCGTCT61600.036.03149849
GTCACCT64250.034.62183429
TATACAT64850.034.4621835
Produced by FastQC (version 0.11.2)