FastQCFastQC Report
Thu 25 Jan 2018
HF7VNBGX5_n01_ko030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHF7VNBGX5_n01_ko030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6643658
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG297350.4475696972962786RNA PCR Primer, Index 38 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC47550.059.08231444
TATGCCG49400.056.94387446
CACCTAG51150.055.35340531
ACCTAGC52250.054.4520832
TCGTATG52100.054.1188243
GTATGCC52550.053.52766845
ATGCCGT52800.053.47797447
TCACCTA53400.052.8915430
GCACACG56250.050.8255511
CTCGTAT54500.050.5931142
ACGTCTG56950.050.07971615
TATCTCG54800.050.05745739
CACACGT58300.049.09753412
ACACGTC59550.047.893213
TAGCTAT60000.047.01847535
GCCGTCT60550.046.4079149
CCTAGCT61750.046.18827433
TGCCGTC61300.046.0080248
GTCACCT62300.045.77844229
CACGTCT62250.045.70544414
Produced by FastQC (version 0.11.2)